{"id":223,"date":"2017-11-08T23:36:38","date_gmt":"2017-11-08T23:36:38","guid":{"rendered":"http:\/\/gero.usc.edu\/labs\/cohenlab\/?page_id=223"},"modified":"2021-11-16T00:14:25","modified_gmt":"2021-11-16T00:14:25","slug":"seahorse-core-facility-gerontology-xf96-analyzer","status":"publish","type":"page","link":"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/","title":{"rendered":"Seahorse Core Facility Gerontology (XF96-Analyzer)"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column][vc_column_text]The Seahorse XF96 is a unique instrument that measures extracellular O2, pH, glucose and lactate fluxes to provide estimates of glucose utilization in cultured cells. The XF96 instrument provides a significant advance over existing methodologies to quantitate cellular metabolic pathways. Unlike radiometric assays, the XF96 does not require radio-labeled substrates, and unlike measurements of cellular oxygen consumption using the classic Clark electrode, it is possible to make high throughput measurements on 96 samples simultaneously in a microplate format.<\/p>\n<p><b>Seahorse XF96 Extracellular Flux Analyzer Service<\/b><\/p>\n<ul>\n<li>Measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR)<\/li>\n<li>Cell types assayed on the XF analyzer: Cell lines, primary cells, isolated mitochondria, worms (<i>C. <\/i><i>elegans<\/i>)<\/li>\n<li>No cleaning is required. All parts that contact cells, media or drugs are disposable<\/li>\n<li>Up to four test compounds (drugs or substrates) may be added automatically to each well. Measurements are performed before and after each compound are added<\/li>\n<li>To make high throughput measurements on 96 samples simultaneously in a microplate format<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_separator][\/vc_column][\/vc_row][vc_row][vc_column][vc_column_text]<\/p>\n<h3><strong>Contact Information<\/strong><\/h3>\n<p><strong>Director:<\/strong> Hemal H. Mehta<br \/>\n<strong>Email:<\/strong> hemal.mehta@usc.edu<br \/>\n<strong>Location:<\/strong> USC Leonard Davis School of Gerontology, 3715 McClintock Ave, B1A, LA, CA 90089<br \/>\n<strong>Phone Number:<\/strong> 213-821-6073<\/p>\n<p><strong>Pricing and Reservations:<\/strong><br \/>\nPlease contact Hemal Mehta by email at hemalmeh@usc.edu<\/p>\n<p><strong>Resources:<\/strong><br \/>\n<a href=\"http:\/\/www.agilent.com\/en\/promotions\/seahorse-xf-technology\">The Seahorse Bioscience Company<\/a>[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>[vc_row][vc_column][vc_column_text]The Seahorse XF96 is a unique instrument that measures extracellular O2, pH, glucose and lactate fluxes to provide estimates of glucose utilization in cultured cells. The XF96 instrument provides a significant advance over existing methodologies to quantitate cellular metabolic pathways. Unlike radiometric assays, the XF96 does not require radio-labeled substrates, and unlike measurements of cellular [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":9,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-full.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-223","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Seahorse Core Facility Gerontology (XF96-Analyzer) - The Cohen Lab<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Seahorse Core Facility Gerontology (XF96-Analyzer) - The Cohen Lab\" \/>\n<meta property=\"og:description\" content=\"[vc_row][vc_column][vc_column_text]The Seahorse XF96 is a unique instrument that measures extracellular O2, pH, glucose and lactate fluxes to provide estimates of glucose utilization in cultured cells. The XF96 instrument provides a significant advance over existing methodologies to quantitate cellular metabolic pathways. Unlike radiometric assays, the XF96 does not require radio-labeled substrates, and unlike measurements of cellular [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/\" \/>\n<meta property=\"og:site_name\" content=\"The Cohen Lab\" \/>\n<meta property=\"article:modified_time\" content=\"2021-11-16T00:14:25+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/cores\\\/seahorse-core-facility-gerontology-xf96-analyzer\\\/\",\"url\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/cores\\\/seahorse-core-facility-gerontology-xf96-analyzer\\\/\",\"name\":\"Seahorse Core Facility Gerontology (XF96-Analyzer) - The Cohen Lab\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/#website\"},\"datePublished\":\"2017-11-08T23:36:38+00:00\",\"dateModified\":\"2021-11-16T00:14:25+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/cores\\\/seahorse-core-facility-gerontology-xf96-analyzer\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/cores\\\/seahorse-core-facility-gerontology-xf96-analyzer\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/cores\\\/seahorse-core-facility-gerontology-xf96-analyzer\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Cores\",\"item\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/cores\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Seahorse Core Facility Gerontology (XF96-Analyzer)\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/#website\",\"url\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/\",\"name\":\"The Cohen Lab\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/gero.usc.edu\\\/labs\\\/cohenlab\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Seahorse Core Facility Gerontology (XF96-Analyzer) - The Cohen Lab","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/","og_locale":"en_US","og_type":"article","og_title":"Seahorse Core Facility Gerontology (XF96-Analyzer) - The Cohen Lab","og_description":"[vc_row][vc_column][vc_column_text]The Seahorse XF96 is a unique instrument that measures extracellular O2, pH, glucose and lactate fluxes to provide estimates of glucose utilization in cultured cells. The XF96 instrument provides a significant advance over existing methodologies to quantitate cellular metabolic pathways. Unlike radiometric assays, the XF96 does not require radio-labeled substrates, and unlike measurements of cellular [&hellip;]","og_url":"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/","og_site_name":"The Cohen Lab","article_modified_time":"2021-11-16T00:14:25+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/","url":"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/","name":"Seahorse Core Facility Gerontology (XF96-Analyzer) - The Cohen Lab","isPartOf":{"@id":"https:\/\/gero.usc.edu\/labs\/cohenlab\/#website"},"datePublished":"2017-11-08T23:36:38+00:00","dateModified":"2021-11-16T00:14:25+00:00","breadcrumb":{"@id":"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/seahorse-core-facility-gerontology-xf96-analyzer\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/gero.usc.edu\/labs\/cohenlab\/"},{"@type":"ListItem","position":2,"name":"Cores","item":"https:\/\/gero.usc.edu\/labs\/cohenlab\/cores\/"},{"@type":"ListItem","position":3,"name":"Seahorse Core Facility Gerontology (XF96-Analyzer)"}]},{"@type":"WebSite","@id":"https:\/\/gero.usc.edu\/labs\/cohenlab\/#website","url":"https:\/\/gero.usc.edu\/labs\/cohenlab\/","name":"The Cohen Lab","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/gero.usc.edu\/labs\/cohenlab\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links":{"self":[{"href":"https:\/\/gero.usc.edu\/labs\/cohenlab\/wp-json\/wp\/v2\/pages\/223","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gero.usc.edu\/labs\/cohenlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gero.usc.edu\/labs\/cohenlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gero.usc.edu\/labs\/cohenlab\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/gero.usc.edu\/labs\/cohenlab\/wp-json\/wp\/v2\/comments?post=223"}],"version-history":[{"count":0,"href":"https:\/\/gero.usc.edu\/labs\/cohenlab\/wp-json\/wp\/v2\/pages\/223\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/gero.usc.edu\/labs\/cohenlab\/wp-json\/wp\/v2\/pages\/9"}],"wp:attachment":[{"href":"https:\/\/gero.usc.edu\/labs\/cohenlab\/wp-json\/wp\/v2\/media?parent=223"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}