Cores

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Aging Biomarker and Service Core

Aging Biomarker and Service Core owns QuickPlex SQ 120 (Meso Scale Discovery, MSD) and SpectraMax M3 multi-mode microplate readers (Molecular Device). The ELISA services include

1) cytokines measurement
2) Alzheimer-related markers (Amyloid beta, phospho-tau) measurement
3) hormones measurement including GH, human and mouse IGF-I& IGFBPs, etc.
4) metabolic biomarkers measurement including insulin, leptin and adiponectin
5) mitochondrial derived peptides (MDPs) measurement including humanin and the other novel MDPs
6) new assay development.

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Seahorse XF-96 Analyzer

The Seahorse XF96 is a unique instrument that measure extracellular O2, pH, glucose and lactate fluxes to provide estimates of glucose utilization in cultured cells. The XF96 instrument provides a significant advance over existing methodologies to quantitate cellular metabolic pathways. Unlike radiometric assays, the XF96 does not require radiolabeled substrates, and unlike measurements of cellular oxygen consumption using the classic Clark electrode, it is possible to make high throughput measurements on 96 samples simultaneously in a microplate format.

The XF Technology:

  • Measures oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) .
  • Completely non-invasive measurement requires no addition of dyes, labels or reporters.
  • Cell types assayed on the XF analyzer: Cell lines, primary cells, isolated mitochondria, worms (C. elegans)
  • Measurements may be repeated multiple times to measure kinetic responses.
  • No cleaning required. All parts that contact cells, media or drugs are disposable.
  • Up to four test compounds (drugs or substrates) may be added automatically to each well. Measurements are performed before and after each compound are added.

Pricing and Reservations:
Please contact Hemal Mehta by email at hemalmeh@usc.edu

Resources:
The Seahorse Bioscience Company